The Effect of Ethanol Extract of Kasturi Citrus (Citrus japonica Thunb.) Peels on Cholesterol-LDL Levels in Male White Rats (Rattus norvegicus) Serum

The effect of ethanol extract of citrus fruit (Citrus japonica Thunb.) peels on LDL
cholesterol levels in serum of white male rat (Rattus norvegicus) has been studied. This
research used 30 white male rat that divided into 6 groups, each group used 5 rats groups
consisting of normal groups, negative group, positive group, and dose group that is dose
250, 500 and 1000 mg/kg BW. The normal group was given Na CMC suspension and
standard feed. The negative group induced high-fat feed, standard feed and suspension of
Na CMC. The positive group induced high-fat feed, standard feed, and atorvastatin
suspension. The treatment group was given high fat feed-induced, standard feed, and
suspension of ethanol extract of citrus fruit. The parameters are LDL cholesterol levels
induced with high-fat feed containing a mixture of 2 mg cow brain and 2 ml duck egg yolk.
The LDL cholesterol levels are calculating by using Assay Homogeneous method with a
photometer (Mindray BA-88) at wavelength 600 nm. The data were analyzed by one way
ANOVA (Analysis of Variance) and followed by the Tukey test. The result showed that
ethanol extract of citrus Kasturi fruit can decrease LDL cholesterol levels by 19.18 % at
doses of 500 and 25.48 % at a dose of 1000 mg/kg BW as indicated by significant
difference with the negative control group (p < 0.05) and there was no significant difference
with the positive group (p > 0.05).